This test paper is made based on the principle of time-resolved fluorescence immunochromatography test. After the sample is added to the sampling hole, it moves along the chromatography membrane with the fluorescent microsphere marker. If there are Brucella immune antibodies in the sample, it will react with the fluorescent microsphere marker. The ball marker combines with the antigen on the detection line T1 to generate a fluorescent signal; if there is Brucella infection antibody in the sample, it combines with the fluorescent microsphere marker and the antigen on the detection line T1 and T2 to generate a fluorescent signal; if Without antibodies present in the sample, no fluorescent signal is produced.